Nucleic Acid Technologies Core

The NAT Core at GTP utilizes cutting-edge sequencing technologies to provide full service for a wide variety of Next-Generation Sequencing (NGS) applications.

The Core is equipped with a complete NGS pipeline consisting of three Illumina sequencers, one NanoPore MinION sequencer, and instruments for NGS sample preps (Covaris ultrasonicater and BluePippin), sample/library QC (Qiaxcel, Bioanalyzer and Qubit), and one liquid handler, the epMotion 5075, for automated pipetting. Serving both discovery and translational research teams at GTP, our team currently processes hundreds of NGS samples every month.

In addition to wet lab services, we routinely perform data analysis and reporting of on and off-target effects of genome editing, plasmid and vector genome sequencing (research and IND-enabling grade). We also work closely with study directors, bioinformaticians and biostatisticians at GTP to support timely in-depth NGS data analysis for complex projects.

Services Performed

  • Design and execution of novel custom NGS workflows
  • Consultation in NGS experimental design, data interpretation, and troubleshooting
  • NGS library prep, QC  and sequencing for:
    • Plasmid sequencing
    • Vector genome sequencing
    • Amplicon sequencing
    • AMP-seq (Anchored multiplex PCR followed by NGS)
    • Whole exome and whole genome sequencing
    • Bulk RNA-seq
  • Data analysis and reporting of indel analysis, on and off-target effects of genome editing, plasmid, and vector genome sequencing
  • Training on nucleic acid quality control (Bioanalyzer, Qubit, Qiaxcel)