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Immunology Core

The mission of the Immunology Core is to analyze and evaluate host immune responses before and after adeno-associated viral vector (AAV) administration, and to gauge its impact on gene therapy treatment.

The Core evaluates humoral and cellular immune responses in body fluids and lymphocytes, respectively. Humoral immune response to AAV capsid is evaluated by an in vitro neutralizing antibody assay. T-cell responses to AAV capsid and to the therapeutic gene are evaluated by cytokine release after specific antigen stimulation.

Body fluids are collected and T cells are isolated from different organs/tissues depending on the route of vector administration and target of gene therapy treatment.

Services Performed

B cell assays

AAV neutralizing antibody assay ( AAV serotype specific)

AAV binding antibody assay (AAV serotype specific)

 

T cell assays

IFNγ ELISPOT assay

Intracellular Cytokine assay

Lymphocyte isolation from blood and tissues

immunology core webpage

Contact Information

Jessica A. Chichester, PhD

Senior Director, Immunology Core

Email: jchich@upenn.edu

Phone: 215-746-6357

Related Publications

Publication 12/05/2018

AAV8 Gene Therapy for Crigler-Najjar Syndrome in Macaques Elicited Transgene T Cell Responses That Are Resident to the Liver.

Molecular Therapy. Methods & Clinical Development

Publication 7/14/2018

Toxicology Study of Intra-Cisterna Magna Adeno-Associated Virus 9 Expressing Human Alpha-L-Iduronidase in Rhesus Macaques.

Molecular Therapy Methods & Clinical Development

Publication 7/14/2018

Toxicology Study of Intra-Cisterna Magna Adeno-Associated Virus 9 Expressing Iduronate-2-Sulfatase in Rhesus Macaques.

Molecular Therapy Methods & Clinical Development

Publication 7/09/2018

Meganuclease targeting of PCSK9 in macaque liver leads to stable reduction in serum cholesterol.

Nature Biotechnology

Publication 6/11/2018

Optimized Adeno-Associated Virus-Mediated Human Factor VIII Gene Therapy in Cynomolgus Macaques.

Human Gene Therapy

Publication 4/01/2018

Assessment of Humoral, Innate, and T-Cell Immune Responses to Adeno-Associated Virus Vectors.

Human Gene Therapy Methods